NAPA anticorps

N° du produit ABIN264392

L’anticorps Souris Monoclonal anti-NAPA a été validé pour WB, IP et EIA. Il convient pour détecter NAPA dans des échantillons de Humain, Rat et Boeuf (Vache).

Aperçu rapide pour NAPA anticorps (ABIN264392)

Antigène: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reactivité: Humain, Rat, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NAPA est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 15D4

Détail du produit anti-NAPA anticorps

Specificité: This antibody specifically recognizes a-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and do not recognize b-SNAP.

Attributs du produit: Synonyms: NAPA, SNAP-alpha, SNAPA, Alpha-soluble NSF attachment protein,N-ethylmaleimide-sensitive factor attachment protein alpha

Purification: Standard chromatographic techniques.

Immunogène: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinanthuman a-SNAP protein and mouse myeloma cells.

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: 0.5-5 μg/mL. Immunoprecipitationon native and denatured samples: 2-10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol without preservatives.

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur NAPA

Antigène: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Autre désignation: SNAP alpha

Sujet: SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (or bSNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas a-SNAP is expressed in all mammalian tissues, b-SNAP is expressed only in brain.Synonyms: Alpha-soluble NSF attachment protein, N-ethylmaleimide-sensitive factor attachment protein alpha, NAPA, SNAP-alpha, SNAPA

ID gène: 8775

UniProt: P54920

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization